Effect of Vit E on myosin light chain kinase activity and expression in the liver of atherosclerosis model rabbit
- VernacularTitle:VitE对动脉粥样硬化模型兔肝脏肌球蛋白轻链激酶活性及表达的影响
- Author:
Huaqing ZHU
;
Bin REN
;
Zhikui JIANG
;
Qing ZHOU
;
Shuyu GUI
;
Yuan WANG
- Publication Type:Journal Article
- Keywords:
myosin light chain kinase;
Vit E;
atherosclerosis;
liver
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effect of Vit E on the MLCK activity and expression in the liver of atherosclerosis model rabbits. METHODS The MLCK activity of rabbit liver was measured by the method of ?- 32P incorporated and its expression was detected by immunofluorescent. RESULTS The model of atherosclerosis was estabilished. After rabbits were fed with cholesterol for four weeks and twelve weeks, the activity of MLCK increased markedly, and there was significantly statistical difference compared with the normal control (P0.05). MLCK expression increased after the rabbits was fed with cholesterol for four weeks, and this increase became more obvious had been the rabbits was fed with cholesterol for twelve weeks. The expression decreased when vitamin E had been added into the cholesterol fed. CONCLUSION The pathology of liver may be associated with the increase of the activity of MLCK. Vit E may reduce MLCK activity and protect hepatocyte from injury.
