Preliminary study on cell-mediated immunity induced by VP_1 gene vaccine against coxsackievirus B_2.
- VernacularTitle:柯萨奇B_2病毒VP_1基因疫苗诱导细胞免疫的初步研究
- Author:
Hong GUO
;
Ye TIAN
;
Xiufen QU
- Publication Type:Journal Article
- Keywords:
Coxsackievirus B 2 Gene Vaccine Cell-mediated immunity
- From:
Chinese Journal of Practical Internal Medicine
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a novel VP 1 gene vaccine against coxsackievirus B 2 and to evaluate the effect of the cell-mediated immunity induced by it.Methods The immunodominant capsid protein VP 1 gene of CVB 2 was amplified by reverse transcript polymerase chain reaction (RT-PCR) and pcDNA 3-CVB 2VP 1was constructed by molecular cloning.The cytotoxic T lymphocyte (CTL) activity was measured by standard 51Cr-release cytotoxicity assay eight weeks after BALB/c mice were immuned by pcDNA 3-CVB 2VP 1.Results The eukaryotic expression vector was pcDNA 3 and subcloning fragment was CVB 2VP 1.The CTL activity of pcDNA 3-CVB 2VP 1 group was higher than that of the control (P
