Relationships between induction of apoptosis by CDDP in Scaber cell and apoptosis-related proteins
- VernacularTitle:顺铂诱导膀胱癌细胞凋亡及其与凋亡相关蛋白的关系
- Author:
Kui WU
;
Gang MENG
;
Yuan WANG
;
Qin ZHOU
- Publication Type:Journal Article
- Keywords:
CDDP;
scaber bladder cancer cell;
bcl 2;
bax;
caspase 3;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of cisplatin on apoptosis in Scaber cell. METHODS The apoptotic cells were detected by TUNEL,HE,eletronic micrpscopy. RESULTS Treatment of Scaber cells with CDDP resulted in characteristics typical of apoptosis. CDDP induced apoptosis of Scaber cells in time and concentration dependent manner. To further investigate the mechanism of apoptosis induced by CDDP, the expressions and activity of apoptosis associated proteins such as bcl 2, bax and caspase 3 were examined using S P method.The results showed: CDDP caused time and concentration dependent decreases in bcl 2 and increased in bax proteins.CDDP bcl 2 and its translocation to perinuclei and nuclei. The expression of caspase 3 in Scaber cell were determined during apoptosis induced by CDDP. CONCLUSION Our investigetion showed that the apoptosis induced by CDDP is related to the increase of bax protein, and the decrease of bcl 2 protein. and its translocation to perinuclei and nuclei.
