The association of the expression level of anti-apoptosis protein p-AKT and gastric mucosal injury induced by indomethacin in mice
- VernacularTitle:磷酸化丝氨酶/苏氨酸蛋白激酶B与吲哚美辛诱导胃黏膜损伤的关系探讨
- Author:
Ming GAO
;
Aiguo TAN
;
Aihua LIU
;
Chunmei ZHAN
;
Cibo HUANG
- Publication Type:Journal Article
- Keywords:
p-AKT;
Indomethacin;
Gastric mucosa;
Anti-apoptosis protein
- From:
Chinese Journal of Rheumatology
2008;12(8):560-562,插三
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of the expression level of anti-apoptosis protein p-AKT and gastric mucasal injury induced by indomethacin in mice.Methods The cytotoxicity induced by indomethacin was measured by LDH assay.The p-AKT expression levels were measured in the gastric mucosal tissues from C57BL/6 mice and rat gastric mucosal (RGM-1) cell lines treated with indomethacin lay western blotting.Results The cytotoxicity induced by indomethacin was in a dose dependent manner.Compared with the control,a typical histological appearance of gastric ulcer was observed in the gastric mucosa of in domethacin-administered mice;p-AKT protein expression in the gastric mucosa of mice and RGM-1 cell lines was decreased after treated with indomethacin.Conclusion The reduction of Anfi-apoptesis protein p-AKT expression may be a new mechanism for the gastric mucosal injury induced by indomethacin.
