Study an mechanism of apoptosis by bortezomib and imatinib in K562 cells in vitro

VernacularTitle:硼替佐米联合伊马替尼诱导K562细胞凋亡机制的研究
Author: Yunxia XIE ; Liangming MA
Publication Type:Journal Article
Keywords: Antineoplastic agents; K562 cells; Livin mRNA; Apoptosis
From: Cancer Research and Clinic 2009;21(4):236-238,241
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of bortezomib alone and in combination with imatinib on apoptosis of human chronic myeloid leukemia cells of the line K562 ,what is more, to explore the mechanism. Methods K562 was cultured and treated with bortezomib and (or) imatinib in different concentrations for 12, 48, 72 hours. Cell proliferation was analyzed by MTT assay, the apoptosis of K562 cells was observed by flow cytometry, and the expression of Livin mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR). Results MTT assay and flow cytometry showed that bortezomib could inhibit K562 cell proliferation and induce its apoptosis, and it can strengthen cells' lethal effect by imatinib. The expression of Livin mRNA was decreased by bortezomib or imatinib to some extent, and was downregulated significantly when combined treatment was given. Conclusion Bortezomib alone and in combination with imatinib can induce K562 cells apoptosis, in which decrease of the expression of Livin mRNA is the possible mechanism.