Characteristic response of CD4~+ cell in chronic Schistosoma japonicum in fection by gene expression profiling
- VernacularTitle:基因表达谱芯片分析日本血吸虫感染慢性期CD4~+细胞的应答特征
- Author:
Minjun JI
;
Chuan SU
;
Xiang ZHU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Antigens,CD4;
T-lymphocyte subsets;
Oligonucle otide array sequence analysis;
Flow cytometry;
Th2 cells
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The changes of cytokine expression on a genomic scale in CD4 + cell of mice c hronically infected with Schistosoma japonicum and their roles in the pathog enesis of schistosomiasis were studied. Methods CD4 + cells were isolated from sp le ens of mice 13 wk infected with Schistosoma japonicum. We used the cDNA micr oarr ay technique to explore the cytokine gene expression profile of CD4 + cells f ro m normal and chronic infected mice. Three-color flow cytometry was performed to study intracellular protein levels of IFN-?、IL-4 of CD4 + cell. Results In the CD4 + cell of chronically infected mice, IL-4 was remarkably increase d at both transcription and protein expression levels (P
