Interleukin-1? up-regulates osteopontin mRNA expression in rat glomerular mesangial cells through the p38MAPK pathway
- VernacularTitle:白细胞介素1?通过p38信号通路上调大鼠肾小球系膜细胞骨调素mRNA的表达
- Author:
Xueqing YU
;
Yongquan LI
;
Xiaoyan LI
;
Shenglang ZHU
;
Xunhua ZHEN
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinase;
Osteopontin;
Mesangial cells
- From:
Chinese Journal of Nephrology
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function role and mechanism of p38 mitogen-activated protein kinase(p38MAPK) in up-regulating osteopontin mRNA expression in rat glomerular mesangial cells induced by interleukin-1?.Methods Activation of p38MAPK was detected with Western blotting,The effect of p38MAPK specific blockade SB203580 on the expression of osteoponlin mRNA in rat mesangial cells induced by interleukin-1? was measured with RT-PCR. Results interleukin-1? could activate p38MAPK in time- and dosage-dependent manner,and up-regulate osteopontin mRNA expression in rat glomerular mesangial cells. SB203580 obviously inhibited the up-regulation of osteopontin mRNA induced by interleukin-1? in dosage dependent manner. Conclusion p38MAPK may play an important role in the up-regulation of osteopontin in rat glomerular mesangial cells induced by interleukin-1?.
