In vitro study on combination of oncolytic adenovirus and Ad - Endo against cervical carcinoma
- VernacularTitle:溶瘤腺病毒和Ad-Endo体外联合作用于宫颈癌细胞的初步研究
- Author:
Weihua YING
;
Guangling CAI
;
Ya MA
;
Guangyin YU
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Cell line,tumor;
Oncolytic viruses;
Endestatins;
Gene therapy
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(9):1417-1419,插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-cervical carcinoma cells effects of oncolytic adenovirus d/1520 and Ad-Endo in vitro,and investigate the influence of d/1520 on the Endostatin expression directed by Ad-Endo in cervical carcinoma cells.Methods After cervical carcinoma cells were infected with d/1520 or/and Ad-Endo,the cytopathic effects (CPE) were observed,and the cytotoxicities were measured by MTT assay.Endestatin concentrations were measured by ELISA.Results d/1520 can replicate in cervical carcinoma cells and induce cytopathie effects; In MTT assay,Ad-Endo hardly inhibited the growth of cervical carcinoma cells,while d/1520 inhibited efficiently the proliferation of cervical cancer cells.And the inhibitory rates in Ad-Endo + d/1520 group were higher than that in d/1520 group.Ad-Endo expressed Endestatin protein in cervical cancer cells,while Endostatin concentrations in Ad-Endo+dl1520 group were much higer than those in Ad-Endo group.Conclusions dl1520 can kill cervical carcinoma cells in vitro,and this eytotoxieity can be enhanced by combining with Ad-Endo.The expression of Endostatin directed by Ad-Endo in cervical cancer cells can be dramatically increased by dl1520.
