IDENTIFICATION OF SPECIFIC ANTIGEN OF TRICHINELLA SPIRALIS MUSCLE LARVAE
- VernacularTitle:旋毛虫肌幼虫特异性抗原的鉴定
- Author:
Zhongquan WANG
;
Jing CUI
;
Deng ZHANG
- Publication Type:Journal Article
- Keywords:
Trichinella spiralis;
Muscle larvae;
Antigen;
SDS-PAGE;
Western blot
- From:
Chinese Journal of Schistosomiasis Control
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find out the specific antigens for immunodiagnosis of trichinellosis. Methods The soluble antigens of Trichinella spiralis muscle larvae were analyzed by SDS-PAGE and Western blot. Results SDS-PAGE revealed that the soluble antigens of T. spiralis muscle larvae had 29 protein bands with molecular weight (MW) from 112 kDa to 12 kDa, among them the protein bands with MW 65,43,42,31,30,20,17,16 kDa were the major bands. Western blot results showed that the protein bands with 112,110,108, 102,97,95,65,63,58,55,53,49,45,43,42 kDa in T.spiralis muscle larval soluble antigens were cross-reacted with sera from rats and patients with paragonimiasis, sera from patients with clonorchiasis, schistosomiasis, and cys-ticercosis. The protein components with 24 - 20 kDa were only reacted with sera from rats, mice infected with T.spiralis and patients with trichinellosis, and not reacted with sera from animals and patients infected with other parasites,and sera from normal rats, mice and healthy persons. Conclusion The protein components with 24-20 kDa in T.spiralis muscle larval soluble antigens are the specific antigen for T.spiralis muscle larvae, it could be applied to the immunodiagnosis and seroepidemiological investigation on trichinellosis.
