Impact of NF-kappaB Activation on TNF-α and IL-1β Expression in Myocardial Ischemia/Reperfusion Injury
10.3870/j.issn.1672-0741.2009.06.008
- VernacularTitle:NF-KB激活对缺血再灌注心肌TNF-α和IL-1β表达的影响
- Author:
Tucheng SUN
;
Xionggang JIANG
;
Kailun ZHANG
;
Zongquan SUN
- Publication Type:Journal Article
- Keywords:
myocardial reperfusion injury;
NF-kappaB;
cytokine
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):752-755,759
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of NF-kappaB activation on TNF-a and IL-1β expression in myocardial ischemia/reperfusion(I/R) injury.Methods Sixty-five Sprague-Dawley rats were randomly divided into three groups:sham,n=5;I/R:30 min of myocardial ischemia followed by 0,15,30,60,120.240 min of reperfusion,n=5 per subgroup;I/R+PDTC:PDTC(15 mg/kg)was given before ischemia,and the time points were the same as those in I/R group.TNF-a and IL-1β mRNA expression was detected by RT-PCR,activity of NF-KB was measured by electrophoretic mobility shift assay (EMSA),and MDA level in myocardium was assayed by TBA method.Results The expression of TNF-a and IL-1β was increased before reperfusion,reached their peak at the time point of reperfusion 30.60 min respectively.and remained high level at the 2nd h after reperfusion.NF-KB was activated 15 min after reperfusion,reached its peak at the first h after reperfusion.In I/R+PDTC group,NF-κB activation was blocked by PDTC.As compared with I/R group,the expression levels of TNF-a and IL-1β were decreased to varying degrees at each time point.and the content of MDA was also reduced in I/R+PDTC group.Conclusion NF-KB activation could play a pivotabrole in the expression of cytokine.Inhibition of NF-κB signal pathway might be a potential therapeutic strategy in reperfusion injury.
