EFFECT OF FGF-10 ON THE SECRETION OF GM-CSF BY NORMAL ADULT HUMAN KERATINOCYTES IN CULTURE
- VernacularTitle:成纤维细胞生长因子10对人角朊细胞表达GM-CSF的刺激作用
- Author:
Xiaobing FU
;
Xiaoqing SUN
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
fibroblast growth factor;
keratinocytes;
granulocyte-macrophage colony-stimulating factor;
cell culture
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of FGF-10 on the secretion of GM-CSF by adult keratinocytes in vitro and to understand the mechanisms involved in the stimulation of granulation tissue formation by FGF-10 during wound healing. Concentrations of FGF-10 used were 4, 16, 125 and 500 ng/ml. Cells were seeded in the amount of 2 500 cells/cm 2 or 5 000 cells/cm 2 in dishes in serum-free medium, and supernatants were collected at 24, 48 and 72 hours after culture. The amounts of GM-CSF in cell culture supernatants were determined using GM-CSF ELISA kits, and cell numbers were counted by haemocytometer. For cells seeded in low density (2 500 cells/cm 2), GM-CSF was not detected at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, the amounts of GM-CSF secreted in cultures with 125 and 500ng/ml FGF-10 were significantly higher than that in negative control (P0.05). At 48 hours, the keratinocytes in the middle area were confluent, and a number of cornified cells were observed, while the productions of GM-CSF in FGF-10 cultures were not higher than that in negative control. There was a clear negative correlation between the secretion production of the growth factor and the total cell number in each dish with a correlated coeffecient of 0.881 (P
