EFFECTS OF MK-801 ON DENTATE GRANULE CELL NEUROGENESIS AFTER DIFFUSE BRAIN INJURY IN THE ADULT RATS
- VernacularTitle:MK-801对成年大鼠弥漫性脑损伤后海马齿状回神经发生的影响
- Author:
Weidong WANG
;
Jincun WANG
;
Yuhua CHENG
- Publication Type:Journal Article
- Keywords:
neurotransmitters;
brain injuries;
NMDA receptor;
glutamic acid
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of selective N-methy-D-aspartate receptor antagonist MK-801 on dentate granule cell neurogenesis after diffuse brain injury in the adult rat, so as to explore the role of glutamic acid on dentate granule cell neurogenesis after diffuse brain injury. 45 adult male SD rats were subjected to diffuse brain injury and randomized into MK-801-treatment group, vehicle-treatment group and control group (n=15 each). By using bromodeoxyuridine ( BrdU ) labelling method and immunohistochemistry to observe dividing cells, the proliferation rates of neural precursor cells in the dentate gyrus(DG) were compared between MK-801-treatment group and corresponding control groups on 2, 4, 6, 8, and 12 days after diffuse brain injury. The results showed that MK-801 (1mg/kg i.p.) significantly reduced the number of BrdU labeled cells in the dentate gyrus on 4, 6, 8 and 12 days after diffuse brain injury (P
