COMPARATIVE STUDY ON HEMATOPOIETIC RECONSTRUCTION EFFECTS OF PERIPHERAL BLOOD STEM CELLS MOBILIZED BY ANTI-CD49d MONOCLONAL ANTIBODY AND rhG CSF IN MICE
- VernacularTitle:抗CD49d单克隆抗体与rhG CSF动员的外周血干细胞移植重建小鼠造血功能的比较研究
- Author:
Zunmin DU
;
Chuanfang LIU
;
Yukun ZHANG
- Publication Type:Journal Article
- Keywords:
peripheral blood stem cell transplantation;
anti-CD49d monoclonal antibody;
granulocyte colony-stimulating factor;
mice, inbred BALB/C
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
The present experiment was to study the hematopoietic reconstruction effects of peripheral blood stem cell (PBSC) mobilized by anti-CD49d monoclonal antibody (McAb) and recombinant human granulocyte colony-stimulating factor(rhG-CSF) in mice. PBSCs from NS-treated mice (control group), rhG-CSF-mobilized mice (experimental group1), and anti-CD49d McAb-mobilized mice (experimental group 2), The changes in respectively, were transplanted to BALB/c mice pre-conditioned with high-dose chemotherapy and total body irradiation white blood cell (WBC) count, four-weeks survival rate, bone marrow nuclear cells (BMNC), granulocyte-macrophage colony-forming units (CFU-GM), and colony-forming unit-spleen (CFU-S) were observed. The results showed that survival rate, WBC, BMNC, CFU-GM, and CFU-S counts were significantly higher in experimental groups 1 and 2 than those of the control group(P
