The secretion of catecholamine in cultured rat adrenal medullary chromaffin cells measured by high-performance liquid chromatography-electrochemical detection assay
- VernacularTitle:原代培养大鼠肾上腺髓质嗜铬细胞分泌儿茶酚胺的高效液相色谱-电化学检测
- Author:
Ling SONG
;
Junmin YANG
- Publication Type:Journal Article
- Keywords:
adrenal medullary chromaffin cells;
catecholamine;
high performance liquid chromatography-electrochemical detection technique
- From:
Chinese Pharmaceutical Journal
1999;(3):172-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the secretion of catecholamine primary cultures of chromaffin cells from rat adrenal medulla were used.METHOD:Catechoalmines(norepinephrine,epinephrine and dopamine) were measured by high-performance liquid chromatography-electrochemical detection technique.RESULTS:Catecholamine released by chromaffin cells with in 20min without slimalus was (73.29±15.32) ng/106 cells.When acetylcholine,nicotine or muscarine was added,the secretion of catecholamine was then increased.CONCLUSION:Using high-performance liquid chromatography-electrochemical detection technique,we can detect sensitively catecholamine released by cultured rat chromaffin cells.
