RECENT DEVELOPMENT IN CLONING OF NEW GENES RELATED TO THE PATHOGENESIS OF VIRAL HEPATITIS
- VernacularTitle:病毒性肝炎发病机制相关基因克隆化的研究策略
- Author:
Jun CHENG
- Publication Type:Journal Article
- Keywords:
hepatitis viruses;
cloning, molecular;
yeast two hybrid
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The pathogenesis of viral hepatitis caused by hepatitis B virus (HBV) and hepatitis C virus (HCV) infection has not been fully elucidated. The cloning of new genes which are related to the hepatitis viruses is an important informational resource for the study of pathogenesis of viral hepatitis. The life cycle, including replication and expression of hepatitis viruses, has been controlled by the protein derived from hepatocyte. The protein-protein interaction between the hepatitis virus and hepatocyte exerts significant impact on signal transduction. The gene expression pattern might be also affected by the expression of hepatitis viral proteins, which might be the mechanism underlying the development of chronic hepatitis and hepatocellular carcinoma (HCC). Yeast one hybrid, yeast two hybrid, DNA chips, suppression subtractive hybridization(SSH), phage display, protein purification and DNA cloning techniques were employed in the present study of the pathogenesis of viral hepatitis, including the study of hepatitis viral DNA/RNA-binding protein identification, hepatitis viral protein-binding protein, and target genes of hepatocyte transactivated by hepatitis viral proteins. All these studies will promote the progress in the field of study on pathogenesis and new therapies of viral hepatitis.
