DEFINITION OF PRE-PRE-S PROMOTER SEQUENCE FROM HEPATITIS B VIRUS GENOME AND IDENTIFICATION OF ITS TRANSCRIPTION ACTIVITY
- VernacularTitle:乙型肝炎病毒基因组中前前-S-编码基因启动子序列的确定及转录活性的鉴定
- Author:
Qian YANG
;
Jing DONG
;
Jun CHENG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
pre-pre-S-region;
promoter
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the promoter activity of pre-pre-S gene, which was found in five clones of hepatitis B virus (HBV) genome from 2 patients with chronic HBV infection, promoter DNA sequence was amplified from HBV-DNA by polymerase chain reaction (PCR).The amplified product was cloned into pCAT3 reporter vector. The HepG2 cells were transfected by pCAT3-pre-pre-S-p. The CAT activity was detected by an enzyme-linked immunosorbent assay (ELISA) kit. It was found that pCAT3-pre-pre-S-p had higher activity of CAT which was 10 fold over that of pCAT3-basic. This result implicated that pCAT3-pre-pre-S-p had promoter activity.
