IDENTIFICATION OF PRE-X PROMOTER SEQUENCE IN HEPATITIS B VIRUS GENOME AND APPRAISAL OF ITS TRANSCRIPTION ACTIVITY
- VernacularTitle:乙型肝炎病毒基因组中前-X-编码基因启动子序列的确定及转录活性的鉴定
- Author:
Qian YANG
;
Jing DONG
;
Jun CHENG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
pre-X region;
promoter
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
It was found that pre-X region was present in five clones of hepatitis B virus (HBV) genome from 2 patients with chronic HBV infection. The open reading frame (ORF) consisted of 168 base pair (bp), and could be transslated with the X gene in frame. To investigate the activity of pre-X gene promoter, promoter DNA sequence was amplified from HBV-DNA by polymerase chain reaction (PCR), The amplified product was cloned into pCAT3 vector, The HepG2 cells were transfected by pCAT3-Pre-X-p. The CAT activity was detected by an enzyme-linked immunosorbent assay (ELISA) kit. It was found that pCAT3-Pre-X-p had higher activity of CAT, which was 3.5 fold over that of pCAT3-promoter. This result implicated that pCAT3-Pre-X-p possessed promoter activity.
