QUANTITATIVE ASSAY AND ANALYSIS OF SPECIFIC ANTIBODIES OF NOVEL CORONAVIRUS IN CONVALESCENT SEVERE ACUTE RESPIRATORY SYNDROME(SARS) PATIENTS
- VernacularTitle:严重急性呼吸综合征康复者冠状病毒特异性抗体定量检测与分析
- Author:
Youning LIU
;
Baoxing FAN
;
Fengying HAO
- Publication Type:Journal Article
- Keywords:
coronavirus;
immunoglobulins;
severe acute respiratory syndrome
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Determination of the specific antibodies of IgG and IgM against SARS virus in serum specimens from convalescent SARS patients. Methods The contents of IgG and IgM antibodies against coronavirus were determined with ELISA in the blood of 22 convalescent SARS patients. At the same time, the sera from 22 healthy people were used as negative control. Results The positive rate of specific IgG was 100.0% in convalescent SARS patients (the minimal OD value was 0.477, the maximal OD value was 1.851, the mean value was 1.163), within 71 days of recovery. The positive rate of specific IgM was 22.73% in convalescent patients (5 in 22 samples), and it became negative in all patients after 65 days. On the contrary, the IgG and IgM antibodies were all negative in all 22 healthy people. Conclusion The results indicated that all patients convalescent from SARS could generate specific IgG antibody, which might last for a long time.
