EXPERIMENTAL STUDY ON MOBILIZATION OF PERIPHERAL HEMATOPOIETIC STEM/PROGENITOR CELLS IN MICE BY A COMBINATION OF CYCLOPHOSPHAMIDE, RECOMBINANT HUMAN INTERLEUKIN-11 AND GRANULOCYTE-COLONY STIMULATING FACTOR
- VernacularTitle:rhIL-11联合rhG-CSF和CTX动员小鼠外周造血干/祖细胞的实验研究
- Author:
Zujun JIANG
;
Fanyi MENG
- Publication Type:Journal Article
- Keywords:
interleukin-11;
human granulocyte colony stimulating factor, recombinant;
cyclophosphamide;
hematopoietic stem cell mobilization
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of recombinant human interleukin 11(rhIL-11), granulocyte-colony stimulating factor (rhG-CSF) combined with cyclophosphamide (CTX) on mobilizing peripheral blood stem/progenitor cells in C57BL/6 mice. A total of 48 C57BL/6 mice were randomized into four groups. CTX (200mg/kg) was injected intraperitonealy on the first day (d0) in the treatment group. rhIL-11 alone or in combination with rhG-CSF was administered subcutaneously, beginning from 24 hours after CTX for 15 consecutive days in two other groups. The changes in peripheral blood white cell counts, platelet counts and the percentage of hematopoietic stem/progenitor cells of the mice were observed. It was shown that rhIL-11 or/and rhG-CSF could increase the numbers of WBC, PLT, CFU-GM, CFU-E, CFU-MK progenitor cells, and the percentage of CD34 positive cells in peripheral blood of myelosupressed mice. The results demonstrated that rhIL-11 alone or in combination with G-CSF and CTX could mobilize bone marrow haematopoietic stem/progenitor cells into peripheral blood.
