SCRAPING METHOD FOR ISOLATION OF RAT PARIETAL CELLS
- VernacularTitle:大鼠壁细胞的黏膜刮取式分离方法
- Author:
Fengpeng SUN
;
Yugang SONG
;
Yan ZHANG
- Publication Type:Journal Article
- Keywords:
rats;
gastric mucosa;
parietal cells, gastric;
cell separation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells.
