PROLIFERATION/APOPTOSIS INDEX——A NEW TOOL IN EVALUATING THE MALIGNANT DEGREE OF GLIOMAS
- VernacularTitle:增殖/凋亡指数——评估胶质瘤恶性程度的新指标
- Author:
Jianqi WANG
;
Guangji ZHANG
;
Yanhua HAN
- Publication Type:Journal Article
- Keywords:
proliferation;
apoptosis;
glioma
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
To establish the proliferation and apoptosis index(P/AI), and to explore its relationship to the malignant degree of gliomas. Specimens of normal brain tissues obtained from 8 patients undergoing decompression operation for head injury, and 69 paraffin-embedded specimens and 26 fresh specimens of the glioma were studied. Immunohistochemical staining of proliferation cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase mediated dUTP DIG nick end labeling(TUNEL) were used to determine the proliferation and apoptosis rates of the glioma. Grading was defined by the ratio between PCNA positive rate and TUNEL positive rate (P/AI). An increase in P/AI denoted an increase in malignancy. It was found that P/AI of normal brain tissue was 0.2-1.5. In 85.1% of grades Ⅰ and Ⅱ gliomas, P/AI was lower than 2.5 and while in 91.7% of grades Ⅲ and Ⅳ gliomas P/AI was 2.5. There were significant differences between normal group, grades Ⅰ and Ⅱ and grades Ⅲ and Ⅳ over gliomas (P
