Effects of Perindopril on amount and mRNA expression of Ca2+ release channels of myocardial sarcoplasmic reticulum in rats with chronic heart failure
10.3969/j.issn.1671-8348.2001.01.006
- VernacularTitle:心衰大鼠心肌SR Ca2+释放通道数量及其mRNA表达的变化及培哚普利干预的影响
- Author:
Zhaohua GENG
;
Longgui LI
;
Jian HUANG
- Publication Type:Journal Article
- From:
Chongqing Medicine
2001;(1):13-14，17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of ACE1 on amount and mRNA expression of Ca2+ release channels(RyR2) of myocardial sarcoplasmic reticulum(SR) in prevention and treatment of chronic heart failure.Methods After the model of chronic heart failure was established with ligation of left corongary artery in rats,the animals were prevented and treated with Perindopril. Hemodynamic parameters, Bmax and Kd of ［3H］－ryanodine binding to RyR2、RyR2 mRNA content were determined.Results Compared with the control group(group C),LVEDP in the heart faliure group (group F)increased(P<0.01),while+dp/dtmax and -dp/dtmaxdecreased significantly(P<0.01).LVEDP was lower but +dp/dtmax and -dp/dtmaxsignificantly higher in the Perindopril treated group(group P)than those in group F(P<0.01).Bmaxof ［3H］－ryanodine binding to RyR2 and mRNA content of RyR2 in group F were lower than those in group C(P<0.01),and these in group P were higher than those in group F(P<0.01). There were no significant difference of Kd among the three groups(P>0.05).Conclusion The amount and mRNA expression of RyR2 of myocardial sarcoplasmic reticulum(SR) decreased in chronic heart failure.Perindopril can improve mRNA expression and amount of RyR2 of myocardial SR in prevention and treatment of chronic heart failure, thus contributing to the improvement of myocardial function.
