Adenovirus mediated transfer of TGF-?1 in treatment of mice autoimmune myocarditis
- VernacularTitle:腺病毒介导的TGF-?1基因转导对小鼠自身免疫性心肌炎的治疗作用
- Author:
Jun ZHANG
;
Qian SHEN
- Publication Type:Journal Article
- Keywords:
transforming growth factor beta;
autoimmune myocarditis;
gene therapy
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study adenovirus mediated transfer of TGF ?1 in treatment of mice autoimmune myocarditis. Methods: Experimental autoimmune myocarditis was induced in BALB/c mice using purified myosin from porcine heart. TGF ?1 was transferred into mice by intravenous injection of adenovirus encoding TGF ?1. Plasma level of myosin autoantibody was detected by indirect ELISA method and plasma level of troponin I was assayed using fluorescence immune assay method. The protective effects of TGF ?1 were evaluated from 3 aspects: (1) the degree of infiltrative inflammatory cells in the heart; (2) the plasma level of myosin autoantibody; (3) the plasma level of troponin I. Results: TGF ?1 successfully ameliorated the myocarditis. Plasma level of troponin I and myosin autoantibody decreased significantly( P
