Experimental study on the mechanism of inducing apoptosis in human SPC-A1 cells by curcumin
- VernacularTitle:姜黄素诱导人肺癌细胞凋亡的作用机制初步研究
- Author:
Dongsheng HUANG
;
Jinhe CHEN
- Publication Type:Journal Article
- Keywords:
curcumin;
SPC-A1;
apoptosis;
cAMP;
Caspase-8
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the mechanism of anti-tumor effects of curcumin on human SPC-A1 cells. METHODS Cell culture、fluorescence microscope、TUNEL method and irradiation immunity techniques were used. RESULTS ①By the action of curcumin, SPC-A1 cells grew against the wall and then peeled off gradually;fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different sizes; The TUNEL method further certified that apoptotic cells reached 42 67% at the concentration of 20 ?mol?L -1 curcumin at 24 h. ②The level of intracellular cAMP concentration in the SPC-A1 cells,treated with curcumin, was significantly higher than that of the control group. ③Treated with 20 ?mol?L -1 curcumin for 24 h, the expression of Caspase-8 mRNA of human SPC-A1 cells was up-regulated. CONCLUSION Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanism may be associated with the rising of the level of intracellular cAMP concentration, and up-regulating expression of Caspase-8.
