The effect of intra-articular injection of CM-chitosan on nuclear factor-κB activation and nitric oxide synthase expression in rat cartilage
10.3760/cma.j.issn.1007-7480.2013.01.010
- VernacularTitle:关节腔注射羧甲基壳聚糖对大鼠骨关节炎软骨核因子-κB活化及一氧化氮合酶表达的影响
- Author:
Wanjun DING
;
Shiqing LIU
;
Weiping TAO
;
Bin HE
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Cartilage;
Nuclear factors;
Nitric oxide synthase;
Carboxymethyl chitosan
- From:
Chinese Journal of Rheumatology
2013;(1):41-45,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of intra-articular injection of CM-chitosan on nuclear factor κB (NF-κB) activation and nitric synthase expression in rat osteoarthritis cartilage,and to explore the mechanism of inhibition of joint destruction.Methods Thirty-six SD rats were randomly divided into three groups:A,B,C,12 in each group.Group A was the sham group,group B,C rats had the medial collateral ligaments cut off and part of medial meniscus were removed to establish osteoarthristis model.Group C rats were injected with 3％ carboxymethyl chitosan intra-articularly 0.15 mg/kg 5 weeks later,and then repeated injection every 1 week.Animals were sacrificed 11 weeks after surgery.The gross changes of cartilage and the expression of NF-κB (P65) were compared by immunohistochemistry,the protein expression of I-κB and P65 in nucleus were detected by Western-bloting.Inducible nitric oxide synthase (iNOS) mRNA and protein expres-sion were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and western blot.The general score of cartilage was analyzed by H test,the rest data was analyzed by one-way ANOVA.Results The cartilage degeneration scores pf group C (intra-articular injection of CM-chitosan group) were significantly less than those of group B.The protein expression of NF-κB of the articular cartilage in group C (106±7)was significantly lower than group B (147±8),the I-κB degradation was inhibited significantly in group C,and the expression of iNOS mRNA and iNOS protein were reduced in OA art~icular cartilage of arthritis rat chondrocytes,therefore,it had protective effect on articular cartilage.Conclusion CMC may inhibit NF-κB signaling pathway by inhibiting the degradation of I-κB in cartilage,which reduces iNOS mRNA and protein expression in rat osteoarthritis cartilage,thereby protects rat osteoarthritis cartilage cells.
