Effects of neferine on gastric carcinoma apoptosis induced by vincristine
- VernacularTitle:甲基莲心碱对长春新碱诱导人胃癌细胞凋亡的影响
- Author:
Shuhong SHI
;
Yingzhi ZHUANG
;
Jianguo CAO
- Publication Type:Journal Article
- Keywords:
neferine;
vincristine;
chemosensitizer;
gastric carcinoma cell;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of neferine on gastric car cinoma apoptosis induced by vincristine in vitro. METHODS Cytotoxicity assay was tested by MTT method. The influence of neferine to affect vincristine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry, AO/EB double fluorescence stain and terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL). RESULTS 2 5, 5,10 ?mol?L -1 neferine enhanced vincristine to inhibit the proliferation of SGC7901 cells; 10 ?mol?L -1 neferine enhanced vincristine (0 1, 0 5, 2, 4 mg?L -1 ) to induce SGC7901 cells apoptosis. CONCLUSIONS Neferine enhanced vincristine to induce gastric carcinoma cells apoptosis. It is inferred a kind of low poisonous and high effective chemosenstizers.
