Role of nuciear factor-?B in the ischemic acute renal failure rat
- VernacularTitle:核因子?B在缺血性急性肾衰竭中作用的实验研究
- Author:
Jianzhou ZOU
;
Xiaoqiang DING
;
Li SUN
;
Jie TENG
;
Yi FANG
- Publication Type:Journal Article
- Keywords:
Kidney failure, acute;
Nuclear factor-?B;
Inducible nitric oxide synthase;
DNA binding activity;
Gel mobility shift assay
- From:
Chinese Journal of Nephrology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the role of nuclear factor-?B(NF-?B) in ischemic acute renal failure (ARF) rats. Methods Gel mobility shift assay was used to detect the DNA binding activity of NF-KB in ischemic ARF rats and reverse transcription-polymerase chain reaction (RT-PCR) assay was used to study the expression of renal inducible nitric oxide synthase (iNOS) . The relationship between DNA binding activity of NF-?B and expression of iNOS was also analyzed. Results The DNA binding activity of NF-?B in renal cortex increased from 1.00 ?0.17 of controls to 3. 67 ? 1. 94 of 6 hours after ischemia-reperfusion ( P
