Adenovirus-mediated transfer of TIMP-4 gene inhibits neointimal formation after balloon injury
- VernacularTitle:腺病毒介导的TIMP-4基因转染抑制血管损伤后新生内膜形成
- Author:
Yanhong GUO
;
Qian LI
;
Guanghui CHEN
;
Jian TANG
;
Wei GAO
- Publication Type:Journal Article
- Keywords:
Tissue inhibitor of matelloproteinases;
Neovascularization, pathologic;
Gene therapy
- From:
Journal of Peking University(Health Sciences)
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of human tissue inhibitor of matelloproteinase 4 (TIMP 4) on vascular smooth muscle cells (VSMCs) migration and the neointimal formation after balloon injury in rats. Methods: The cultured VSMCs were transfected with an adenoviral vector containing human TIMP 4 gene, AdTIMP 4. Effect of TIMP 4 on VSMC migration was investigated by monolayer cell scrape. AdTIMP 4, control adenoviral vector or PBS was transduced into the rat carotid artery from the adventitial after carotid artery injury. Cell number within the internal elastic lamina 4 days after gene transfer was counted and neointima/media area ratio 28 days after gene transfer was calculated. Results: The migration distance of VSMCs infected with AdTIMP 4 was inhibited markedly. Morphometric analysis demonstrated a statistically significant reduction in the number of cells migrated into neointima compared with controls [(32.5?4.8) cells per section, (33.8?7.0) cells per section and (8.2?2.4) cells per section for uninfected, AdGFP treated and AdTIMP 4 treated arteries, respectively]. There was a reduction of intima/media ratio of TIMP 4 treated group by 66.5% compared with control groups 28 days after gene transfer ( P
