CHANGES IN LEVELS OF CYTOKINES SECRETED BY THE PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH HEART FAILURE AND INHIBITORY EFFECTS OF CAPTOPRIL ON CYTOKINES PRODUCTION
- VernacularTitle:心力衰竭患者单核细胞细胞因子水平的变化及卡托普利对其抑制作用的研究
- Author:
Guodong JIA
;
Fengzhi WANG
;
Guoshu LIU
- Publication Type:Journal Article
- Keywords:
heart failure, congestive;
monocytes;
cytokines;
captopril
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the changes in levels of tumor necrosis factor ? (TNF ?), interleukin 6 (IL 6) and interleukin 1? (IL 1?) produced by the peripheral blood mononuclear cells (PBMC) of patients with congestive heart failure (CHF), and to investigate the effects of captopril, one of angiotensin converting enzyme inhibitors in active form, on production of these cytokines. PBMC from 35 CHF patients and 12 healthy controls were isolated by density centrifugation with Ficoll Hypaque, and were cultured with captopril at 10 -4 ～10 -2 mol/L in the presence of lipopolysaccharide (LPS). After incubation for 24 hours, TNF ? and IL 6 contents in the culture supernatants were measured by an enzyme linked immunosorbent assay (ELISA), and IL 1? by radioimmunoassay (RIA). The results showed that PBMC from CHF patients released significantly more TNF ?, IL 6 and IL 1? compared with cells from controls ( P
