Correlation of cardiac AT_1 receptor and ?_1 receptor with PKC/MAPKs signal transduction pathway in myocardial remodeling.
- VernacularTitle:心肌重塑中AT_1受体?_1受体与PKC和MAPKs信号转导通路间关系的探讨
- Author:
Yuansheng LIU
;
Jihong GUO
- Publication Type:Journal Article
- Keywords:
Myocardial remodeling Receptor Signal transduction pathway Gene
- From:
Chinese Journal of Practical Internal Medicine
2002;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To inquire into the correlation of cardiac AT 1 receptor,? 1 receptor with PKC/MAPKs(protein kinase C/mitogen-activated protein kinase) signal transduction pathway during myocardial remodeling.Methods Twenty-four Wistar rats were randomly divided into four groups-sham group,infarcted group,losartan group and betaloc group.Except sham group,left anterior descending coronary artery was ligated for 21 days.In the other three groups,expression changes of cardiac collagen subtype Ⅰ,Ⅲ,fibronectin(FN),c-fos,ERK 1 (extracellular signal-regulated kinase,ERK)and PKC proteins were studied with immunohistochemistry and computer image analysis. c-fos mRNA expression was tested by reverse transcription and polymerase chain reaction(RT-PCR).Results Expressions of collagen Ⅰ,collagen Ⅲ , FN,c-fos,ERK 1 and PKC proteins were significantly enhanced(P
