The experimental study on MR diffusion and perfusion-weighted imaging during permanent focal cerebral ischemia in rabbits,comparison with the changes of intracellular Ca~(2+) in brain slice
- VernacularTitle:兔脑缺血区扩散、灌注成像及细胞内钙的对照研究
- Author:
Fang ZHANG
;
Jingxia XIE
;
Hongbin HAN
- Publication Type:Journal Article
- Keywords:
Magnetic resonance imaging;
Model, animal;
Diffusion;
Cations, divalent;
Calcium
- From:
Chinese Journal of Radiology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Calcium overload has important effect on cell injury in the ischemic stroke MR DWI/PWI mismatch may display the ischemic penumbra We try to explain the relationship between the characters of MR DWI/PWI and intracellular Ca 2+ in the different ischemic region through the model of focal cerebral ischemia Methods Twenty-eight rabbits underwent permanent middle cerebral artery occlusion (MCAo) From 0 5 h to 36 h after the onset of occlusion, diffusion-, perfusion-, and T 2-weighted MR imaging were performed to generate ADC, CBF, CBV parameters and anatomic images After the MR scanning, the same brain slices were incubated with Fluo-3/AM followed by the examination using laser confocal scanning microscope (LCSM) At different ischemic time, fluorescence intensity (FI) of the intracellular Ca 2+ in the DWI/PWI match region and mismatch one was recorded FI match /FI contralateral and FI mismatch /FI contralateral were produced and analyzed Results (1) MR outcome: The DWI/PWI match was located in the lateral caudoputamen and the DWI/PWI mismatch was mainly located in the frontoparietal cortex At 0 5 h after MCAo, CBF and CBV of lateral caudoputamen and frontoparietal cortex in the affected side deceased marked and the rCBF and rCBV of lateral caudoputamen were less then that of frontoparietal cortex in all groups ( t =-11 74, P
