SIGNAL PATHWAY INVOLVED IN HEAT-STRESSED FIBROBLASTS
- VernacularTitle:离体热损伤成纤维细胞所涉及的细胞信号通路
- Author:
Biao CHENG
;
Xiaobing FU
;
Zhiyong SHENG
- Publication Type:Journal Article
- Keywords:
mitogen-activated protein kinases;
wounds and injuries;
signal transduction;
fibroblasts
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the activities of signal transduction pathway involved in heat-stressed fibroblast in vitro. Human dermal fibroblasts were cultured in Dulbecco′s modified Eagle′s medium with 5% calf serum at 5%CO 2 in a water-saturated atmosphere. Cultured cells were heated to 45℃ for 10min. Western blotting was used to detect ERK1/2 and JNK expression, and the expression of caspase-3 protein was observed by immunoflurescence technique. The results showed that the MAPKs signal transduction pathway was activated in heat-stressed fibroblasts. The expression of JNK reached the peak at 60min, then maintained up to 180min. The expression of ERKs peaked at 30min, then lowered. The expression of caspase-3 was weak at 30min after heat-stress, and became evidently strong at 60min. The signal pathway of ERKs and JNK played important roles in the changes in biologic characteristics of fibroblasts after heat-stress.
