Application of NanoString nCounter System in biomedical fields

VernacularTitle:NanoString数字基因定量技术在生物医学领域的应用进展
Author: Dong HU ; Fang WANG ; Wei ZHOU ; Jiaxi DUAN ; Lingling FAN ; Shiang HUANG
Publication Type:Journal Article
Keywords: NanoString; Digital gene analysis; Multiplexed gene quantitation; Biomedical fields
From: International Journal of Biomedical Engineering 2013;36(6):351-356,封3
CountryChina
Language:Chinese
Abstract: NanoString nCounter Analysis System is a newly developed gene expression detection platform that directly measures multiplexed mRNA levels through digital counting of individual mRNA transcripts.This technology uses as little as 100 ng of RNA and can obtain accurate gene quantitative data from up to 800 genes in one reaction.It requires no reverse transcription,enzymes and amplifications,and its sensitivity and accuracy are comparable to real time quantitative PCR.NanoString technology has been more and more extensively used in frontiers of biomedical research and clinics such as in validation of data from high-throughput platforms,gene expression profiling,gene regulatory network,molecular subtyping,diagnosis and prognosis of diseases.