Determination of 3-methylhistidine and tyrosine in incubated buffer and checking samples by HPLC with PITC pre-column derivation
- VernacularTitle:异硫氰酸苯酯衍生法测定孵育液中的3-甲基组氨酸与酪氨酸
- Author:
Fangnan LIU
;
Li TAN
;
Nan LUO
;
Wei ZHOU
- Publication Type:Journal Article
- Keywords:
Methylhistidine;
Tyrosine Liquid;
Chromatography;
Incubated buffer
- From:
Parenteral & Enteral Nutrition
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To determine 3-methylhistidine(3-MH) and tyrosine(Tyr) in standard solution and preparations by high performance liquid chromatography(HPLC) with phenylisothicyanate(PITC) pre-column derivation. Methods:PITC was used as pre-column derivative of HPLC and stored in refrigerator after drying.Two phases gradient elution was performed with acetic sodium and cyanide for 50 minites. Results:The linearity of the measurement was very good for 3-MH and Tyr(r= 0.999 1 and 0.999 2).The recovery rate was 82.6% and 87.6%,respectively. Conclusions:The method of HPLC with PITC pre-column derivation for determination of 3-MH and Tyr is stable,efficient,and sensitive.PITC is an ideal derivative.
