Effect of chitosan on PKC activity and DAG concentration in murine peritoneal macrophages
- VernacularTitle:甲壳胺体外对小鼠巨噬细胞PKC和DAG的作用研究
- Author:
Dongsheng LIANG
;
Mingchun LI
;
Fengmei AN
;
Yuejun WANG
;
Jinhua YUAN
- Publication Type:Journal Article
- Keywords:
chitosan;
macrophages;
protein ki- nase C;
diacyl glycerol:signal transduction
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT AIM To investigate the effect of ch-itosan on protein kinase C (PKC) activitives and diacyl glycerol(DAG)concentration in murine peritoneal macrophages. METHODS A new ion-pair reversed-phase high liquid chromatography and ra-dioimmunoassay were used to determinate the activity of PKC and the concentration of DAG respectively. RESULTS ( 1) Chitosan induced activation and translocation of PKC in murine peritoneal macrophages. The peak time was 25 min and the activity of PKC came back the basic level at 1. 5 h. (2)Chitosan increased the production of DAG in murine peritoneal macrophages. The peak time was 30 s and the concentration of DAG came back the basic level at 3 min. CONCLUSION The im-munopoteniating effect of chitosan may be associated with the channel of DAG /PKC.
