Effects of triptolide on endothelin-1 and its gene expression in lung of asthmatic guinea-pigs
- VernacularTitle:哮喘豚鼠内皮素-1及其基因表达和雷公藤内酯醇的影响
- Author:
Qing HAN
;
Chuansheng GE
;
Li FEI
- Publication Type:Journal Article
- Keywords:
triptolide;
asthma;
ET-1;
receptor;
ECE,RT-PCR
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore the effects of trip-tolide on endothelin-1 and its gene expression in lung of asthmatic guinea-pigs. METHODS The contents of ET-1 in lung of asthmatic guinea-pigs were measured with radioimmunoassay, and the expressions of ET-1, ETA, ETB and ECEmRNA with reverse transcription DNA polymerase chain reaction (RT-PCR). RESULTS 0 All of the indexes were increased after guinea-pigs were induced asthma. At the 4th hour after first induction of asthma, ET-1 reached the top, but at the 2rd hour, ET-1mRNA, ETAmRNA and ECEmRNA were markedly higher than control group (P
