Determination of Norcantharidin using high performance liquid chromatography
- VernacularTitle:去甲斑蝥素片的高效液相色谱质量检测
- Author:
Hongzhi GAO
;
Xiuguo ZHANG
;
Ying HUANG
;
Junhua GUO
- Publication Type:Journal Article
- Keywords:
norcantharidin;
determine;
HPLC
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT AIM To determine Norcantharidin in its tables. METHODS A PHLC method was adopted, using Polaris C18 column(5?,4.6?250 mm) and UV detector at 211 nm wavelength. The mobile phase is consisted of water: methanol(85 : 15 V/V), adjust the pH to 3.1 with phosphoric acid. RESULT Excellent liner relationship was ob- tained from the range of 25 to 1 000 mg ? L-1. The minimal limit of Norcantharidin was 0.2 mg ? L-1. Recovery was 100.84% and RDS was 1.335%. COUCLUSION Practice suggested that this method was rapid, accuracy and suitable for Norcantharidin determining.
