Literature and bioinformatic analysis of dysregulated genes in lung tissues of hyperoxic lung injury
10.3760/cma.j.issn.1673-4408.2013.06.028
- VernacularTitle:基于文献挖掘的高氧损伤肺组织相关基因的生物信息学分析
- Author:
Yuge HUANG
;
Shandan ZHANG
;
Hao HAN
;
Xing CHEN
;
Haiping YIN
;
Xuehui ZHENG
- Publication Type:Journal Article
- Keywords:
Hyperoxia;
Lung injury;
Computational biology;
Lung tissue
- From:
International Journal of Pediatrics
2013;40(6):639-641,654
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the pathogenesis mechanism of hyperoxic lung injury and the effective means for its clinical treatment,the difference of the gene expressions between lung tissues of hyperoxic lung injury and normal lung was compared.Methods The differentially expressed genes between lung tissues of hyperoxic lung injury and normal lung were obtained from PubMed.The dysregulated genes in lung tissues of hyperoxic lung injury were analyzed by a series of bioinformatics methods,including pathways,gene ontology and functional annotation clustering analysis.Results 467 lines of differentially expressed genes were found and genes more than 2-fold regulated were 189.We sought the mapping of genes in the KEGG databases through functional annotation tools,and we discovered there were 5 lines of pathways with difference having outstangding statistical significance through metabolic pathways enrichment degree analysis.It reflected the pathways were closely related to hyperoxic lung injury (the 2-fold upregulated genes were 14,the 2-fold down-regulated genes were 6).GO analysis revealed that these genes were involved in hematopietic cell lineage,axon guidance,adherens junction,T cell receptor signaling pathway and focal adhesion.Conclusions Therefore,it is believed that the above-mentioned 20 lines of gnes are the major ones for the hyperoxic lung injury and the research on them will provide effective means for revealing the molecular mechanism of hyperoxic lung injury and identifying the targeted therapy.
