Effect of baicalein on high glucose-induced expression of extracellular matrix and transforming growth factor ?1 in proximal tubular epithelial cells
- VernacularTitle:黄芩素对高糖诱导近端肾小管上皮细胞外基质及转化生长因子?1表达的影响
- Author:
Wen SHEN
;
Fuming LU
;
Yong GU
;
Shanyan LIN
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Baicalein;
Protein kinase C;
Transforming growth factor ?1;
Type Ⅳ collagen;
Renal tubular epithelial cells
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of baicalain on high expression of ECM and TGF-?1 in proximal tubular epithelial cells cultured in high glucose concentration. Methods LLC-PK1 cells were divided into six groups: (1)normal glucose group(NG, 5. 5 mmol/L D-glucose), (2)high glucose group(HG, 25 mmol/L D-glucose), (3) HG + PKC inhibitor(10?mol/L chelerythrine chloride), (4)HG + baicalein(50 ?mol/L), (5) HG + baicalein(100 ?mol/L), (6) HG + baicalein (200 ?mol/L) . PKC activity was detected. Expression of ColⅣ, FN and TGF-?1 was examined by in situ hybridization and immunocytochemistry(ABC) techniques. Results At concentrations of 100 ?mol/L and 200 ?mol/L, baicalein decreased membranous PKC activity in LLC-PK1 cells by 42% and 68% , respectively ( P
