Prediction and identification of linear B-cell epitopes in major group 3 aller-gen derived from Dermatophagoides farina
- VernacularTitle:粉尘螨3类变应原的B细胞线性表位预测及鉴定
- Author:
Na LI
;
Chaopin LI
;
Jidong DIAO
;
Beibei ZHAO
;
Wei LU
;
Yuxin JIANG
- Publication Type:Journal Article
- Keywords:
Dermatophagoides farina;
Major group 3 allergen derived from Dermatophagoides farina(Der f 3);
B cell epit-ope;
Epitope prediction;
Identify
- From:
Chinese Journal of Schistosomiasis Control
2014;(3):296-299,307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To predict and identify the linear B-cell epitopes in the major group 3 allergen derived from Derma-tophagoides farina(Der f 3). Methods The linear B-cell epitopes of Der f 3 allergen were analyzed based on the physicochemi-cal properties of amino acids including antigenicity,surface accessibility,flexibility,hydrophilicity,beta-turn by online bioinfor-matics softwares. The eight predicted peptides of linear B-cell epitopes were artificially synthesized and incubated with three aller-gic serum pools(4 serum samples in each),which were consisted of total 12 serum samples from the allergic individuals,and the strong positive epitopes were selected. Results Eight B-cell epitopes from Der f 3 were predicted successfully. Five of eight B-cell epitopes were identified with strong IgE-binding abilities followed by specific IgE assay. The amino acid sequences of them were following:33KAKAGDCP40, 86HASGGEKIQVAEIYQHENYDSMTID110, 118LKTPMTLDQTNAKPVPLPPQGSDVKVG144, 156QEGSYSLP163 and 199DVANGGVDSCQGDSGGPVVD218. Conclusions Five linear B-cell epitopes of Der f 3 allergen have been identified successfully. This result might provide a basis of the diagnosis and treatment for asthma.
