Lack effect of metabotropic glutamate receptors on neurotoxicit y of PC12 cells induced by 6-OHDA
- VernacularTitle:Ⅱ、Ⅲ组亲代型谷氨酸受体激动剂对6-羟基多巴诱导的PC12细胞毒性无保护作用
- Author:
Changhong MENG
;
Jianhua DING
;
Gang HU
- Publication Type:Journal Article
- Keywords:
pharmacology;
metabotropic g lutamate receptors;
PC12 cells;
6-hydroxydopamine;
glutamate;
cell viability
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore if metabotropic glutamate receptors (mGluRs) i nduces neuroprotection against 6-hydroxydopamine (6-OHDA) neurotoxicity in PC1 2 cells. METHODS: The alteration of the glutamate in extracellul ar fluid of PC12 cells was detected by high performance liquid chromatography (H PLC) with a fluorescent detector. The cytotoxic activity of PC12 cells was assay ed by means of MTT colorimetric method. RESULTS: 6-OHDA dose-d ependently increased glutamate release and decreased cell activity of PC12 cells , and the mGluRs ligands had no significant effect on that. CONCLUSION: The mGluR3 ligands has no protective effect on neurotoxicity of PC12 cel ls induced by 6-OHDA.
