A STUDY OF DISTRIBUTION IN THE TISSUES AND LONG-TERM TOXICITY OF THERAPEUTIC DNA VACCINE IN MICE
- VernacularTitle:治疗型HBV DNA疫苗在小鼠组织中的分布和长期毒性研究
- Author:
Guoyu MO
;
Guangming CHEN
;
Zhiyin HUANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
vaccines, DNA;
toxicity tests
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the safety of the therapeutic DNA vaccine against the hepatitis B virus. Plasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids combined with electrotransfer in doses of 10 and 50?g. In long term toxicity study, HBV DNA vaccine was administered by repeated intramuscular injections combined with electrotransfer of pDNA to NIH mice in doses of 30, 60 and 120?g, twice a week for four consecutive weeks. Morbid manifestations, behaviors, hematology, blood chemistry, anti nuclear antibodies, and histopathology were analyzed. Results showed that plasmid DNA was detected primarily in the muscle at the site of injection, where it remained for up to 8 weeks. Eight repeated intramuscular injections of HBV DNA vaccine showed no adverse effects on behaviors, hematology, blood chemistry, and histopathology. No evidences of autoimmune mediated pathology and anti nuclear antibodies were observed in the mice. These results suggested that the therapeutic HBV DNA vaccine was safe and well tolerated.
