INDUCTION OF IMMUNE RESPONSES AND SAFETY PROFILES IN RHESUS MACAQUES IMMUNIZED WITH THE THERAPEUTIC DNA VACCINE AGAINST HEPATITIS B VIRUS
- VernacularTitle:治疗型HBV DNA疫苗肌注电转染法免疫恒河猴的效果及安全性观察
- Author:
Guoyu MO
;
Guangming CHEN
;
Fuqin YANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
vaccines, DNA;
immun ization;
transfection
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the immune response and safety of the therapeutic DNA vaccine against the hepatitis B virus in rhesus macaques immunized by intramuscular injection of therapeutic DNA vaccine combined with electroporation. HBV DNA vaccine was administered to rhesus macaques by repeated intramuscular injections combined with electroporation in doses of 0 2,1 and 2mg, monthly for three consecutive months. The anti HBs antibody level was used to evaluate the efficacy of induction of humoral immunity. After that, the vaccine was intramuscularly injected daily in the same dose for six days to evaluate its safety. It was found that immunization with the therapeutic DNA vaccine against hepatitis B virus by means of intramuscular injections combined with electroporation induced a strong and specific anti HBs humoral immune response in rhesus macaques.Nine repeated intramuscular injections of the vaccine did not show adverse effects on behaviors, hematology, blood chemistry, and histopathology. No evidences of autoimmune mediated pathology and anti nuclear antibodies were observed in the rhesus macaques. These results suggested that electroporation significantly improves the efficacy of the therapeutic HBV DNA vaccine in rhesus macaques.The vaccine is safe and well tolerated.
