THE ROLE OF TELOMERASE OLIGODEOXYNUCLEOTIDE IN THE INHIBITION OF GROWTH OF MALIGNANT GLIOMA CELLS
- VernacularTitle:端粒酶反义寡核苷酸对恶性脑胶质瘤细胞生长的抑制作用
- Author:
Jianqi WANG
;
Guangji ZHANG
;
Yanhu HAN
- Publication Type:Journal Article
- Keywords:
glioma;
telomerase;
oligonucleotides
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the curative effect of antisense telomerase ODN on malignant glioma cells. The activity of telomerase in 16 fresh malignant gliomas was detected positive by PCR ELISA method. After covered with lipofectin, the 5?mol/L telomerase ODN was incubated with cultured gliomas cells. PCNA, TUNEL positive rate and the phase of cell cycle were detected by FcM per 24h. The results showed: Anti sense ODN inhibited telomerase activity after being applied for 48 hours, and inhibition was complete at 72 hours, The ODN inhibited proliferation of malignant gliomas and promoted their apoptosis markedly at 72 hours. After 96 hours, most transfected glioma cells were stopped to grow at G 2/M phase, and the number of apoptosis cells increased as time went on. The results suggested that anti sense telomerase ODN could inhibit the telomerase activity, and in turn promote aptoptosis of glioma cells and inhibit the growth of the tumor.
