Oridonin induced HeLa cell apoptosis through capase pathway
- VernacularTitle:冬凌草甲素通过激活caspase诱导HeLa细胞凋亡
- Author:
Chunling ZHANG
;
Lijun WU
;
Shinichi TASHIRO
;
Satoshi ONODERA
;
Takash IKEJIMA
- Publication Type:Journal Article
- Keywords:
oridonin;
HeLa cell;
apoptosis;
caspase
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the mechanisms of oridonin-induced HeLa cell apoptosis. METHODS Morphological observation, agarose gel electrophoresis, LDH release and caspase inhibitors were used. RESULTS Oridonin had significant apoptotic effect on HeLa cells in a dose- and time-dependent manner. The marked morphological changes including condensed chromatin, nuclear fragmentation and apoptotic bodies and DNA ladder in agarose gel was observed. The activity of caspase-3 was increased about 2 times of control value after treatment with oridonin. CONCLUSION Oridonin induces HeLa cell apoptosis through caspase pathway.
