Effects of chloride channel blockers on the contractile and relaxative responses in rat aorta rings
- VernacularTitle:氯通道阻断剂对大鼠主动脉环收缩和舒张反应的影响
- Author:
Quan YI
;
Jian LIANG
;
Yandong WANG
;
Guiping ZHANG
- Publication Type:Journal Article
- Keywords:
chloride channel blockers;
thoracic aorta;
phenylephrine(PHE);
ATP
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of DIDS and furosemide on the contraction induced by phenylephrine and the endothelium-dependent relexation induced by ATP. METHODS Measurement of isometric force of rat thoracic aortae rings with and without endothelium. RESULTS DIDS(1～300 ?mol?L -1 ) and furosemide(10～320 ?mol?L -1 ) inhibited the contractile response induced by phenylephrine in a concentration-dependent manner. The inhibitory rate was different between aortic ring endothelium-intact and that endothelium-denuded. The values of IC_ 50 for DIDS were (12.0?8.0) ?mol?L -1 and (28.3?7.3) ?mol?L -1 respectively,while the values of IC_ 50 for furosemide were (17.9?6.6) ?mol?L -1 and (41.0?15.6) ?mol?L -1 respectively. DIDS(10 ?mol?L -1 ) did not chang ATP-induced vasodilative effect at the concentrations of 10 ?mol?L -1 and 100 ?mol?L -1 , but enhanced the relaxation induced by 1 mmol?L -1 ATP. Furosemide (20 ?mol?L -1 ) had no effect on the relaxation induced by ATP(10 ?mol?L -1 ), but prolonged the relaxation when the concentration of ATP added to 100 ?mol?L -1 and 1 mmol?L -1 ( P
