Determination of paeonol in the bulk drug and injection by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定原料药及注射剂中丹皮酚的含量
- Author:
Yancai SUN
;
Yuxian SHEN
;
Guoping SUN
;
Liming CHEN
;
Jian QU
;
Wei WEI
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
paeonol;
content
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To determine the concentration of paeonol in the bulk drug and injection, a method of reversed-phase high performance liquid chromatography (RP-HPLC) was developed. METHODS Lichrospher C_ 18 column(4.6 mm?250 mm, 5 ?m)was used with methanol-acetonitrile-water(30∶40∶30)as mobile phase at a flow rate of 0.8 ml?min -1 . 20 ?l of sample was injected into the C_ 18 column where the temperature was 25℃. The detection wavelength was 274 nm. RESULTS The linear regression equation for paeonol was = 3 772.525 8 X- 0.747 4 (r = 1.000 0, n =5) under the linearity range from 0.02 mg?L -1 to 25.60 mg?L -1 . The average recovery was 99.87%. The relative standard deviations of the intra-day and inter-day were less than 4%. CONCLUSION The method is simple and rapid, which may be used for the determination of paeonol and its preparation for different purpose.
