RECONSTRUCTION OF A COMPOSITE SKIN SUBSTITUTE WITH COLLAGEN SPONGE AS DERMAL SCAFFOLD
- VernacularTitle:海绵状胶原膜作为真皮支架构建复合皮的研究
- Author:
Jun YANG
;
Shichu XIAO
;
Zhaofan XIA
- Publication Type:Journal Article
- Keywords:
epidermis;
dermis;
collagen sponge membrane;
skin tissue engineering
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To reconstruct a composite skin substitute composed of keratinocytes and collagen sponge as dermal scaffold. Collagen was extracted from fresh porcine skin. After being mixed with chondroitin sulfate, it was frozen and dried under vacuum to form a spongy membrane. Keratinocytes were separated from the foreskin with the routine method, and they seeded on the collagen membrane. The keratocytes which were carried by the collagen sponge were cultured, and the growth and proliferation were observed. The result showed that keratinocytes could grow and proliferate to form a confluent layer after 2～3 weeks. It indicated the the collagen sponge membrane showed no toxicity to human epidermal cells, and it could be used as a dermal scaffold in the construction of a composite skin substitute.
