EXPERIMENTAL STUDIES ON SKIN FIBROBLASTS TRANSFECTED WITH HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR GENE

VernacularTitle:血管内皮细胞生长因子基因转染皮肤成纤维细胞的实验研究
Author: Gang LI ; Zhaofan XIA
Publication Type:Journal Article
Keywords: vascular endothelial cell growth factor; transfection; fibroblasts; skin tissue engineering
From: Medical Journal of Chinese People's Liberation Army 1983;0(05):-
CountryChina
Language:Chinese
Abstract: This study was aimed to improve the therapeutic results of composite skin substitutes. Human vascular endothelial cell growth factor (hVEGF 165 ) gene was constructed to an eukaryotic expression vector pcDNA3. Transfection of recombinant vector pcDNA3 hVEGF 165 into human dermal fibroblast cells was performed. VEGF protein level in the supernatant of transfected fibroblasts culture was determined. Its biological activities were tested by observing the growth rate of the human umbilical vein endothelial cells/HUVEC after being stimulated with the said supernatant, and by performing the Miles assay in guinea pigs. The results showed that these transgenic cells were able to secrete VEGF to certain extent, with biological activities to enhance the growth of HUVEC in vitro and improve vascular permeability. It indicated that transgenic fibroblasts could resurface the dermal substitute of a composite skin.