COMPARISON OF GROWTH FACTOR mRNA EXPRESSIONS IN HUMAN SKIN FIBROBLASTS BETWEEN TWO AND THREE-DIMENSIONAL CULTURE SYSTEMS
- VernacularTitle:二维和三维培养的人皮肤成纤维细胞中生长因子mRNA表达的比较研究
- Author:
Zhiguo LIU
;
Gang LI
;
Wan LIU
- Publication Type:Journal Article
- Keywords:
collagen gel;
cell culture;
fibroblast growth factor
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To compare the growth factors mRNA expressions in human skin fibroblasts cultured in two (2D) or three dimensional systems (3D). Human skin fibroblasts were isolated and cultured in collagen jelly (3D) or the regular culture plates (2D). After 72 hours, the total RNA of fibroblasts were extracted. Specific oligonucleotide primers for the growth factor VEGF and bFGF were synthesized and reverse transcriptase polymerase chain reaction (RT PCR) was utilized to amplify the mRNA of those growth factors. RT PCR productions were separated by electrophoresis in 1% wt/vol agarose gels and photographed. Semi quantitative data were statistically analyzed. Although the morphology of fibroblasts in 3D culture was different from that in 2D culture, the RT PCR analysis revealed the same expression and concentration of VEGF mRNA and bFGF mRNA in human skin fibroblasts cultured in different systems.
